This method determines acid detergent fibers, which are residues after digestion with H2SO4 and CTAB. Fiber residues are mainly cellulose and lignin. Demonstration: This method is suitable for cereals, feed, forage and all fibre-containing materials.

_1. Analytical balance - 0.1 mg can be weighed.

_2. The oven can keep the temperature of 102 2 C.

_3. The digestion apparatus can be digested at 100 (+0.5 C) and maintain a pressure of 10-25 psi. The instrument must be able to create similar flow samples to ensure uniformity of extraction (ANKOM2000, 65rpm agitation, ANKOM technology).

4. Filter bag - consisting of chemical inert and heat-resistant filter media, can be heat-sealed and retain 25 micron particles (F57 and F58, ANKOM Technology) while maintaining solution permeability.

Heating Sealer - Enough to seal filter bags to ensure complete closure (1915, ANKOM technology).

Desiccant foldable sealed bag with desiccant to remove air from the surrounding of the filter bag (MoistureStop weighing bag, ANKOM technology).